For two weeks I've been getting a practical up and running for next Monday. First one of the reagents was off and it made the results look good when they weren't. I found it, fixed it, tried again and got good results. So I made up more of the samples to run and checked them - not working. Went back and used original samples again -now they're not working....argh.
I'm going to try once more under really careful conditions with every control I can think of. If it still doesn't work, I may be scrounging round for Monday.
There goes my weekend.
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